Cloning and Expression Analysis of MYB Transcription Factor Gene in Sweet Cherry
Wei Hairong
Zong Xiaojuan
Zhu Dongzi
Tan Yue
Xu Li
Chen Xin
Wang Jiawei
Liu Qingzhong
Abstract：Based on our previous transcriptome data of sweet cherry fruit, the MYB transcription factor gene was cloned using RT-PCR method from sweet cherry cultivar'Tieton',and named PaMYB10.The se-quence was submitted to GenBank with the accession number of ALH21137.1.Sequence analysis revealed that the deduced amino acid of PaMYB10 contained R2 and R3 conserved domain and it was a typical R2R3-MYB family gene.The encoded protein PaMYB10 had the closest genetic relationship with Prunus persica, Prunus domestica and Prunus mume.The result of qRT-PCR showed that PaMYB10 was expressed in stem, young leaf,flower and fruit tissues in red cultivar'Tieton',but the expression quantity was different.The ex-pression quantity of PaMYB10 was the highest in the mature fruit of 'Tieton'.The content of anthocyanin in'Tieton' increased gradually and the expression level of PaMYB10 showed significantly up-regulated along with the fruit development.However, the content of anthocyanin and expression level of PaMYB10 in the yellow cultivar'13-33' were almost unchanged and remained at a very low level during fruit ripening.In the later stage of fruit development,the content of anthocyanin and expression level of PaMYB10 in the red culti-var'Tieton' were significantly higher than those of the yellow cultivar '13-33'.It is speculated that the high level expression of PaMYB10 may associated with the anthocyanin accumulation in red sweet cherry fruit.
Keywords：Sweet cherryAnthocyaninTranscription factorMYBGene expression
Publication Date：2018-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 6-11 )