Cloning and Sequence Analysis of Pod Shattering Resistant Gene SHAT1-5 from Cultivated and Wild Soybeans in Shandong Province
Lin Yanhui
Li Wei
Zhang Yanwei
Liu Yun
Wang Caijie
Xu Ran
Zhang Lifeng
Abstract：Pod shattering is one of the important factors affecting soybean yield.Therefore,the genetic mechanism of pod shattering trait in cultivated soybean has become a hot spot at domestic and overseas.In this research,the cultivars including Shanning 11,Shanning 17,Qihuang 34 and Lu 0305-1,and the wild germplasms including Dongying wild soybean 1 and 2 and Shandong wild soybean 1 and 2 were used as experimental materials.According to the previous reports,we cloned a pod shattering resistant gene SHAT1-5 through PCR amplification,and analyzed its DNA polymorphism.The results showed that SHAT1-5 appeared different multiples of TAA repeats at 1599 bp location in Shandong cultivated and wild soybeans,respectively.The key regulation region of about 4 kb in upstream of SHAT1-5 promoter was extremely conservative,and no deletion of 20 bp was found in Shandong cultivated soybean.These results are different from the previous reports,indicating that the pod shattering trait of Shandong cultivated soybean and wild soybean may be regulated by other genes.
Keywords：Cultivated soybeanWild soybeanPod shatteringGenePolymorphism analysisPromoter
Publication Date：2018-01-31
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )