Genetic Diversity Analysis of Daye Tea Germplasm in Yunnan Based on EST-SSR Markers
Shang Weiqiong
Duan Zhifen
Yang Yijian
Li Youyong
Cheng Hao
Liu Jie
Yang Xingrong
Yang Shengmei
Yi Bing
Liu Benying
Bi Xiaoqing
Abstract：Abundant genetic variation is very important for improving environmental adaptability and genetic improvement of crop.In order to know the genetic diversity of Daye tea germplasm resources in Yunnan,94 tea germplasms were selected for the analysis using 28 pairs of SSR primers.A total of 128 distinctive loci were detected with the mean as 4.57;the Shannon's information index (Ⅰ) ranged from 0.91 to 1.66 with the mean as 1.30,and 0.91 and 1.66 were in the loci 5087 and 1969 respectively;the polymorphism information content (PIC) ranged from 0.476 to 0.753 with the mean as 0.64,and 0.476 and 0.753 were also in the loci 5087 and 1696 respectively;the average observed heterozygosity and the average expected heterozygosity were 0.71 and 0.70 respectively.The results of genetic differentiation analysis and cluster analysis for the source areas of 94 tea germplasms showed that genetic variation mainly existed between the germplasms from different source areas but not from the same source area,and most germplasms from the same source area distributed in different groups;there was no significant correlation between the genetic distance and the geographical distance.It was concluded that the cluster analysis and the analysis of PIC had the same results,which showed abundant genetic diversity.These results could provide research foundations for breeding,innovation and utilization of Yunnan tea germplasms.
Keywords：YunnanDaye teaEST-SSRMolecular marker
Publication Date：2018-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 16-22 )