System Optimization for Inducing Callus and Buds from'Xiaobaixing'(Prunus armeniaca)

Xie Xiaoting; Guo Mingxing; Xu Zhuye; Dai Lijuan; Hao Fengshun; Feng Jianrong

doi:10.14083/j.issn.1001-4942.2017.12.002

Abstract：To establish rapid propagation and regeneration system for'Xiaobaixing'in vitro and provide foundations for genetic transformation,with embryo and leaf as explants,we studied the effects of disinfection with mercuric chloride and sodium hypochlorite on seed and embryo,the effects of different concentration ratios of 2,4-D(0.5,1.0,1.5 mg/L),ZT(0.5,1.0,1.5 mg/L)and TDZ (1.0,2.0,3.0 mg/L) on embryo induction,and that of 2,4-D (0.5,1.0 mg/L),IBA (0.5,1.0 mg/L) and NAA (0.5,1.0 mg/L) on leaf induction.The results showed that the pollution rate and mortality were the lowest with 10％ sodium hypochlorite disinfecting seed and embryo for 10 minutes,which were 2.6％ and 0.7％ respectively,and the survival rate was the highest as 96.7％.In the treatment condition of 3/4MS containing with 2.0 mg/L TDZ and 1.0 mg/L 2,4-D,the rate of callus formation and adventitious bud induction with embryo as explant were the highest,which were 98％ and 96％,respectively.In the treatment condition of 3/4MS containing with 2.0 mg/L AgNO3,1.0 mg/L ZT,2.0 mg/L TDZ and 1.0 mg/L IBA,the rate of callus formation and adventitious bud induction with leaf as explant were the highest,which were 96％ and 93％ respectively.In conclusion,the callus differentiation to adventitious buds with embryo as explant was better than that with leaf as explant.The best treatment for disinfecting seed and embryo was both with 10％ sodium hypochlorite for 10 minutes.The optimal medium for inducing callus redifferentiation to adventitious buds of embryo was 3/4MS containing with 2.0 mg/L TDZ and 1.0 mg/L 2,4-D,while that for leaves was 3/4MS containing with 2.0 mg/L AgNO3,1.0 mg/L ZT,2.0 mg/L TDZ and 1.0 mg/L IBA.