Phenotypic Analysis of Peanut Semi-Dwarf Mutant sdm1 and Its Response to Gibberellin
Li Huanni
Qiu Jingjing
Ma Junjie
Li Changsheng
Hou Lei
Abstract：One semi-dwarf mutant sdm1 with stable inheritance was isolated from peanut cultivar Shanhua 13 induced by fast neutron irradiation.Its dwarf morphology,economic traits and response to gibberellin were analyzed.The results showed that compared with Shanhua 13,sdm1 showed semi-dwarf in plant height,thicker branches,larger seeds and darker seed coat color.The seed oil content of the mutant was increased and the protein content was decreased.The GA3 content in the leaves of sdm1 mutant was determined using enzyme-linked immunosorbent assay (ELISA),and we found that its gibberellin content was significantly lower than that in Shanhua 13.Treated with exogenous GA3 could restore the dwarf phenotype of mutant.The qRT-PCR was used to analyze the transcriptional level of genes related to GA biosynthesis pathway and signal transduction pathway,and the results showed that the expression level of AhGA2ox,AhGA3ox,AhGA2Oox,AhDella2 and AhDella3 changed significantly.The changed expression of these key enzyme genes led to the decrease of gibberellin content in mutant plants and caused dwarf phenotype.
Keywords：PeanutSemi-dwarfMutantGibberellinsGene expression
Publication Date：2017-12-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )