Analysis on Production Condition and Chemical Composition of Toxin in Pestalotiopsis clavispora
Feng Youren
Liu Baosheng
Bai Penghua
Abstract:To deeply reveal the pathogenesis of Pestalotiopsis clavispora,the toxin production conditions were optimized,the contents of protein and soluble sugar in toxin filtrate were measured,and the toxin chemical compositions were analyzed by GC-MS.The results showed that the toxin production ability varied significantly under different conditions.The best toxin production conditions were Czapek liquid culture medium with pH value of 7.0,25 ℃,day alternates with night,continuously shaking for 25 days with the rotational speed of 120 r/min.Moreover,the protein content of toxin was 180 μg/mL.The soluble sugar content of toxin was 2 057.25 μg/mL.The chemical compositions of toxin were tripropoxysilane,decamethyl cyclopentasiloxane,dodecamethyl cyclohexasiloxane,1,4 dimethoxy-6,7,8,9 tetraheptone,glutarate,methyl carbamate.
Keywords:Pestalotiopsis clavisporaToxinToxin production conditionGas chromatogram and mass spectraChemical composition
Publication Date:2017-01-01
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:7( 81-87 )
Shandong Agricultural Sciences

Shandong Agricultural Sciences

ISTIC
ISSN:1001-4942
Year, Vol.(Issue):2017,49(10)