Detection of Wx-mq Gene for Low-Amylose Content by ARMS-Tm-shift-qPCR Technology
Chen Feng
Xu Jiandi
Jiang Mingsong
Zhang Quanfang
Zhu Wenyin
Li Guangxian
Zhou Xuebiao
Yang Lianqun
Abstract：Wx-mq gene is a low amylose trait controlled gene and closely relates with the eating quality of rice.According to the rice low amylose starch content control gene Wx-mq No.497 position existing G ＞A single nucleotide variants,two real-time PCR genotyping methods,ARMS and Tm-shift based on SYBR Green Ⅰ,was combined to establish the fluorescence real-time quantitative PCR system,which could accurately distinguish the GG homozygote,AA homozygote and GA heterozygosis of Wx-mq gene No.497 position.The experiment results showed that the ARMS-Tm-shift real-time PCR genotyping method based on SYBR Green Ⅰ did not need probe synthesis with simple designs.It was a rapid,simple and specific genotyping method.It could be used in high-throughput genotyping of Wx-mq gene in molecular marker assisted breeding.
Keywords：RiceWx-mq geneLow-amylose contentARMS-Tm-shift-qPCR
Publication Date：2017-10-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 15-19 )