Cloning and Bioinformatics of TiAP1 Gene from Thinopyrum intermedium
Tian Qiuju
Song Jing
Yang Yanlin
Xu Tian
Wang Honggang
Feng Deshun
Abstract：The wheat-Thinopyrum alien addition line SN6306 was originated from the intergeneric hybridization between YN15 and Thinopyrum intermedium (Host) Nevski.Through PCR amplification,the full length cDNA of TiAP1 gene was obtained.Its open reading frame (ORF) was 1 272 bp and encoded a peptide of 423 amino acids.The BlastP analysis results showed that it belonged to the aspartic protease family.The bioinformatics analysis results showed that TiAP1 protein contained a signal peptide,whose cleavage site was located between the 16th and 17th amino acid,and two xylanase inhibitor domain structures,which located in the N and C end respectively.The TMHMM predicting results showed that the protein had no transmembrane helix structure.So the protein was not secreted protein.The phylogenetic tree showed that TiAP1 gene was similar to the corresponding gene in Triticum urartu Thum.ex Gandil and Aegilops tauschii.These results would provide the reference for the research of TiAP1 gene functions.
Keywords：Wheat-Thinopyrum alien addition lineTiAP1Gene cloneBioinformatics analysisPowdery mildew
Publication Date：2017-10-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 1-9 )