Gene Expression and Stress Resistance Analysis of AhSOS2 Transgenic Rice

Li Yingxiu; Zhang Guojia; Wang Yingying; Wang Qingguo; Wang Yifan; Li Zhen; Pan Jiaowen; Liu Wei

doi:10.14083/j.issn.1001-4942.2017.09.004

Abstract：SOS2 (salt overly sensitive 2) is an important class of salt tolerance related genes,which is known to play important roles in the processes of regulation of intracellular ion balance,stress response and adaption of plants to salt damage.In our previous research,AhSOS2 (GenBank accession number as HG797656) had been cloned from peanut leaves.Bio-informatic analysis showed that it belonged to SOS2 gene family and encoded 446 amino acids,which belonged to serine/threonine protein kinase.In this research,four AhSOS2 transgenic rice lines named SOS2-1,SOS2-2,SOS2-3 and SOS2-7 were obtained.Fluorogenic quantitative RT-PCR analysis showed that AhSOS2 expressed in all the four transgenic lines,and the expression level in SOS2-3 was the highest.After treating with 250 mmol/L NaCl,the physiological indexes of transgenic rice lines such as relative electronic conductivity,contents of proline and MDA,activities of antioxidant enzymes such as SOD,POD,CAT and APX were detected.The results showed that under stress conditions,the relative electronic conductivity of transgenic lines were lower than that of control,and the activities of antioxidant enzymes related to stress response were higher than that of control.It indicated that AhSOS2 had certain functions in promoting the resistance abilities to salt and stress conditions of acceptor materials.This research laid a solid foundation for further study of function and resistance mechanism of AhSOS2 and SOS2 gene family.