Effects of Cephalosporin and Kanamycin on Rooting of Peanut Tissue Culture Seedlings
Ruan Jian
Guo Feng
Li Xinguo
Shi Lingmin
Tian Haiying
Zhang Hui
Wan Shubo
Peng Zhenying
Abstract：Resistance screening is an indispensable step in Agrobacterium mediated genetic transformation of peanut,but the use of antibiotics have significant influence on peanut seedling growth and rooting.In order to make clear the sensitive concentration of antibiotics on rooting of peanut seedlings,the peanut cultivation variety Fenghua 1 tissue culture seedlings were used as experimental materials,and the culture media with different concentrations of kanamycin or cefotaxime were used to detect the peanut seedling rooting rate and growth status.The results showed that 0~250 mg/L cefotaxime had little effect on peanut growth,while kanamycin had significant effect on peanut seedling rooting.0~10 mg/L kanamycin has no obvious effect on peanut seedling rooting and growth,but under the condition of 10 mg/L kanamycin,the seedlings had no branch roots,what''s more,15 mg/L kanamycin significantly inhibited the elongation of roots.When the rootless plantlets were transfered into MS medium after cutting off the bottom of the seedlings,the roots grew out again,whereas the rootless plantlets did not grow roots if not cutting off the bottom.After screening with 75 mg/L kanamycin,the rooting rate of the kanamycin-resistant tissue culture plantlets could reach 31%.This study could provide certain theoretical supports for roots induction during the late stage of peanut genetic transformation.
Keywords：CefotaximeKanamycinPeanutTissue culture seedlingRooting rateGenetic transformation
Publication Date：2017-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 33-37 )