Application of Oligo-Nucleotide Fluorescence in situ Hybridization(FISH) in Identifying Wheat-Aegilops uniaristata Germplasm
Gong Wenping
Li Jianbo
Li Haosheng
Song Jianmin
Liu Aifeng
Cao Xinyou
Han Ran
Cheng Dungong
Zhao Zhendong
Yang Zujun
Liu Cheng
Liu Jianjun
Abstract：So far,the cytogenetic approaches for the identification of wheat-Aegilops uniaristata germplasm are lacking.In the present research,Fluorescence in situ hybridization(FISH)using Oligo-pTa535-1 and Oligo-pSc119.2-1 as probes,and FISH using(GAA)8 as probe are performed on wheat-Ae.uniaristata amphiploid and additions.As a result,a standard FISH pattern was established for the identification of all chromosomes of wheat and Ae.uniaristata simultaneously.Meanwhile,chromosome translocations 5BS.3BS and 5BL.3BL were detected in the self-pollinated 1N addition progenies,and oligo-nucleotide sequence deletion was also detected on chromosome arm 5NL of Ae.uniaristata in the self-pollinated 5N addition progenies,providing important material for researching the relationships between chromosome structure variation and phenotypic changes.Moreover,a spontaneously formed wheat-Ae.uniaristata 4N(4B)substitution line was identified by the established FISH karyotype from the self-pollinated 4N addition progenies,making a solid foundation for further inducing wheat-Ae.uniaristata chromosome translocations with chromosome 4N involved.
Keywords：Aegilops uniaristataFluorescence in situ hybridizationChromosome structure aberrationSubstitution line
Publication Date：2017-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 12-18 )