Clone,Bioinformatic Analysis and Protein Expression of CyPD Gene in Peach Fruits
Shangguan Xiangchao
Hu Shunqing
Zhou Jie
Zhu Shuhua
Abstract：Mitochondrial membrane permeability transition pore(MPTP)is a kind of protein complex playing important roles in cell apoptosis and necrosis.Cyclophilin D(CyPD)plays roles in cell mitochondria-mediated apoptosis,which is an important part of MPTP.However,there are fewer researches on CyPD in plants.In this study,the full-length cDNA of CyPD was gained by molecular biology method.It was 663 bp,contained 615 bp of the coding sequence and encoded a polypeptide of 204 amino acids.The recombinant CyPD protein was expressed in Escherichia coli.The phylogentic tree revealed that CyPD was more closely related to apple CyPD protein in evolution.The three-dimensional structure of CyPD showed that the protein contained 2 α-helix and 8 β-pleated sheets.These results would lay a foundation for studying the structure and function of CyPD protein further.
Keywords：Feicheng peachMitochondriaCyPDGene cloneProtein expression
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 7-11 )