Preparation of Rice Phytochrome B Antibody and Its Specificity Analysis
Yan Lihua
Lu Nannan
He Yanan
Yin Haibo
Xie Xianzhi
Abstract：Phytochrome(PHY)is one of the important photoreceptors in plant.Phytochrome family mainly senses red and far-red lights.Rice phytochrome gene family is composed of three members known as PHYA,PHYB,and PHYC.Our previous studies has suggested that PHYB plays important roles in regulating rice growth and development as well as stress responses.However,absence of effective PHYB antibody greatly hinders the dissection of mechanisms of PHYB functions in rice.In this study,we selected the peptide specific to PHYB protein by comparing the amino acid identity among three rice phytochrome members.The cDNA fragment encoding specific PHYB peptide was isolated and cloned into prokaryotic expression vector pET16b.The PHYB antigen was obtained after isopropyl thio-beta-d-galactose(IPTG)induction and purification of expressed protein.The polyclonal PHYB antibody was prepared by immunizing New Zealand rabbits.Western blotting assays revealed that PHYB polycolonal antibody prepared in this study was specific to detect PHYB protein in rice extracts.The results from this study were necessary for comprehensively exploring the functional mechanism of PHYB in rice.
Keywords：RicePhytochromeProkaryotic expressionAntibody
Publication Date：2017-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )