Construction and Sequence Analysis of Complete cDNA Clone of SD Virulent Strain of Newcastle Disease Virus
Yuan Xiaoyuan
Yang Jinxing
Zhang Yuxia
Meng Kai
Wang Youling
Ai Wu
Abstract：The whole genomic cDNA sequence of SD virulent strain of Newcastle disease virus was amplified in sections, and each fragment was attached to the clone vector pBR322 by In-Fusion technology.The whole genomic cDNA clone was obtained.Through the fragmented-PCR amplification and sequencing analysis, the results showed that the whole genomic cDNA clone of SD virulent strain was successfully constructed and the T7 promoter sequence was successfully imported.The identity of amino acid sequence between the whole genomic cDNA clone and parent strain was 99.9%.Single sequence alignment showed that there were 11 base mutations in the whole genomic cDNA clone, and only 2 sites caused the change of amino acid sequence.However, because of no occurrence of deletion and insertion of genes in the whole sequence, the number of full-length amino acids had no change.The successful construction and sequence analysis of whole genomic cDNA clone of SD virulent strain laid the key technical foundations for future reverse genetic operation.
Keywords：Newcastle disease virusWhole genomic cDNARecombinant plasmidSequence analysis
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 12-15 )