Cloning and Functional Identification of Peanut AhDGAT1 Promoter
Zhang Hui
Zheng Ling
Wan Shubo
Li Xinguo
Guo Feng
Shan Lei
Gao Wenwei
Peng Zhenying
Abstract：Diacylglycerol acyltransferase (DGAT) is a rate-limiting enzyme in the biosynthesis pathway of triacylglycerol (TAG).In this study, with peanut cultivar Luhua 14 as material, we cloned the promoter sequence of AhDGAT1 gene (pAhDGAT1) by genome walking method.By bioinformatics analysis, we found that the pAhDGAT1 had many TATA-box, CAAT-box, light regulation, stress and defense response and hormone response elements.The constructed pAhDGAT1∶GUS plant expression vector was transformed into tobacco leaves by Agrobacterium tumefaciens-mediated method.By GUS staining,we found the GUS enzyme activity were detected in all organs of the positive transgenic tobacco lines, while in stigma, anther and young seed, the expression levels were higher than those in filament and style.It indicated that pAhDGAT1 was not tissue-specific promoter and had constitutive promoter feature.
Keywords：PeanutAhDGAT1 genePromoterGUS stainingFunction identification
Publication Date：2017-07-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 1-6,11 )