Establishment and Optimization of Regeneration System for Virus-Free Shoot Tip of Phalaenopsis amabilis
Zhu Jiao
Ma Lei
Liu Fang
Yang Yuanjun
Zhang Jihua
Pan Haijun
Dong Daofeng
Abstract：The shoot tip of Phalaenopsis amabilis was cultured as explant to study the effects of inorganic salt concentration, sugar concentration, hormone proportion and explant size on survival rate and virus-free effect of shoot tip growing point.The results showed that when the explant had the size of 0.2 to 0.4 mm and attached 1 leaf primordium, the ORSV could be eradicated.When the inorganic salt and sugar concentration decreased properly, the survival rate of shoot tip growing point could improve.When 15 g/L soft sugar was added into culture medium, it was beneficial to survival and growth of shoot tip.The medium containing 1/3 MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 NAA was suitable for shoot tip culture of Phalaenopsis amabilis.
Keywords：Phalaenopsis amabilisVirus freeTip shootRegeneration
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 60-63 )