Germplasm Innovation and New Variety Breeding in Orange Leafy Head Chinese Cabbage (Brassica rapa L.ssp.pekinensis)

Zhang Yihui; Li Huayin; Wang Fengde; Li Jingjuan; Ding Qian; Liu Lifeng; Sun Lingqiang; Xu Shaojun; Kong Tao; Wang Cuihua; He Qiwei; Gao Jianwei

doi:10.14083/j.issn.1001-4942.2017.05.003

Abstract：In this research,the molecular breeding was used to innovate germplasm and select new varieties in orange leafy head Chinese cabbage (Brassica rapa L.ssp.pekinensis).We cloned the candidate key gene BrCRTISO,which coded carotenoid isomerase controlling synthesis of orange pigments in leafy head.Differences in structure of BrCRTISO gene between orange and white leafy head Chinese cabbage were also analyzed,in which,one SNP(C952-T952) marker and one InDel marker Bror-intron1 closely linked with orange leafy head were developed.New good pure self-incompatible lines of orange leafy head germplasm had been innovated by molecular marker assisted selection,of which,08428,08460,08468 and 08469 were better than others.These four lines were applied to make cross combinations,from which,new orange leafy head varieties Tianzhengjuhong 65 and Tianzhengjuhong 62 were obtained.Planting and disease resistance tests for several years (2010-2016) showed that these two new varieties had strong disease resistance,and their growth periods were short (55 ～60 days),which were suitable for being cultivated in spring or autumn.The β-carotene content of Tianzhengjuhong 65 reached 26.163 mg/gDW,11.15 times of common Chinese cabbage.In addition,the content of soluble solids was also higher than other Chinese cabbage varieties,which owned good taste.In 2014,Tianzhengjuhong 62 was approved to be Chinese National Protected Geographic Indication Product (No.13066669) in Taian,China.In 2015,Tianzhengjuhong 65 was approved by both National and Beijing Crop Variety Approval Committee.Meanwhile,Tianzhengjuhong 62 was approved by Beijing Crop Variety Approval Committee.In 2016,Tianzhengjuhong 65 was approved by Shandong Crop Variety Approval Committee.This paper would be useful to other plant molecular breeding because of our successful breeding work.