Differential Proteomics Analysis on Apple in Response to Continuous Cropping Obstacle

Ni Wei; Ni Weiru; Zhao Jing; Wang Zenghui; Mao Zhiquan; Chen Xuesen; Shen Xiang

doi:10.14083/j.issn.1001-4942.2017.05.001

Abstract：This research aims to explore the mechanism of apple in response to continuous cropping obstacle at the protein expression level by confirming the differential expression proteins of different apple cultivars answering continuous cropping.Stronger replanting-tolerant cultivar Fuji 2001 and weaker replantingtolerant cultivar Shoufu 1 were grown in pots with replanted soils.Under the same cultivation and management conditions,label-free technology was used to identify the differential expression proteins in the leaves of apple which had different resistance abilities to continuous cropping.The differential expression proteins were compared with GO,COG and other databases to analyze their function and classification.The results showed that 43 kinds of differential or particular expression proteins were identified from two apple cultivars,and were mainly divided into four categories,including one protein related to photosynthesis,seventeen proteins referring to energy and carbohydrate metabolism,eight proteins related to plant defense and resistance,and seventeen proteins with other functions.There were great differences among the proteins of energy and carbohydrate metabolism and plant defense and resistance.Different apple cultivars had different protein expression levels related to continuous cropping.Furthermore,the stronger replanting-tolerant apple cultivar had higher protein expression level than the weaker one.It was inferred that the allergen proteins and pathogenesis related proteins,which joined in defensive reaction,were the key proteins of apple in response to continuous cropping.The stronger replanting-tolerant apple cultivar enhanced various different metabolic pathways to strengthen the resistance to continuous cropping obstacle and reduce the harm to plants.