Genetic Diversity Analysis of Wheat Lines from Dryland Regional Trials

Chen Xiangping; Song Chanyuan; Li Ximei

doi:10.14083/j.issn.1001-4942.2017.03.004

Abstract：Regional trial is an important step during variety certification and popularization of wheat.The regional trial material can reflect the level and development tendency of wheat breeding to some extent.The genetic diversity analysis of 40 wheat lines from dryland regional trials in 2014 was conducted in this study.The results showed that 113 allelic variations were detected by 42 SSRs.The average genetic richness (2.69),genetic diversity (0.27),and polymorphic information content (0.22) all showed that the population genetic diversity level was relatively low.The genetic diversity analysis among the three subgenomes showed that the average genetic richness was arranged in the order of A ＜ B ＜ D,while the genetic diversity and polymorphic information content were both arranged in the order of A ＞ B ＞ D.The genetic diversity analysis among the seven homoeologous groups showed that the average genetic richness was arranged in the order of 7 ＜ 3 ＜ 1 =4 ＜6 ＜ 5 ＜ 2,while the genetic diversity and polymorphic information content were both arranged in the order of 3 ＜ 7 ＜ 2 ＜ 4 ＜ 5 ＜ 6 ＜ 1.Integrating the three indexes,the population genetic diversity level had no obvious difference among the three subgenomes or the seven homoeologous groups.The genetic similarity index among the 40 lines reached up to 0.43,which indicated that the genetic difference was relatively little and the genetic relationship was relatively close.The genetic similarity index between 40 lines with Lumai 21 and Qingmai 6 was 0.45 and 0.46 respectively,which were both higher than that between tested lines with Jimnai 22 (0.41).The cluster analysis showed that there was no obvious subpopulations in the tested population.In conclusion,this study had some guidance meaning for the dryland wheat breeding in Shandong Province.