Differential Analysis of Genes Coding for Cyclic-Nucleotide Gated Channels in Two Chinese Cabbage Inbred Lines
Liu Shuantao
Zhang Zhigang
Li Qiaoyun
Wang Xiao
Wang Lihua
Zhao Zhizhong
Wang Shufen
Xu Wenling
Liu Xianxian
Liu Chen
Abstract：In plants,cyclic-nucleotide gated channels (CNGCs) are non-selective cation channels encoded by a large gene family.They can uptake and transport mnost cations such as Na +,K + and Ca2+,among which,Ca2+ plays pivotal roles in the physiology and biochemistry of all plants.Calcium deficiency often causes tip-burn in Chinese cabbage.Different genotypes of Chinese cabbage have obvious differences in the sensitivity to tip-burn.In this study,re-sequencing was conducted in two genotypes of Chinese cabbage inbred lines,He102 and 06-247,which showed significant differences in tip-burn reaction.Six genes coding for CNGCs were screened out and showed coding-region differences between two lines.They were Bra003081,Bra022702,Bra032132,Bra024067,Bra021265 and Bra031529,which showed homologous to Arabidopsis CNGC1,4,6,9,19,20 respectively.The potential transmembrane domains were predicted.These CNGCs contained 5 ～8 transmembrane domains and their C-terminals were all located in the inside of cell membrane.Amino acid sequence alignment showed that,Bra003081 and Bra031529,which mutated in 06-247,showed C-terminal large fragment deletion and small fragment elongation respectively;Bra032132 and Bra024067,also mutated in 06-247,showed only 2 to 3 amino acid insertion or deletion in C-terminal region;the mutation of Bra021265 and Bra022702 appeared in Hel02,in which,Bra021265 was alternative-splicing mutation while Bra022702 was single amino acid insertion/deletion mutation in N-terminal region.All the mutations of CNGCs in 06-247 appeared in the C-terminal region,which might influence their functions.The results provided clues for studying gene function of CNGCs,developing site-specific markers and exploring their relations to tip-burn in Chinese cabbage.
Keywords：Chinese cabbageTip-burnCyclic-nucleotide gated channels (CNGCs)Gene mutation
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 10-15 )