Molecular Marker-Assisted Selection for Resistance to Turnip mosaic virus in Chinese Cabbage (Brassica campestris L.ssp.pekinensis)

Li Qiaoyun; Zhang Zhigang; Zhao Zhizhong; Liu Shuantao; Wang Lihua; Gao Huichao; Li Yizhen; Xu Wenling; Liu Xianxian; Liu Chen

doi:10.14083/j.issn.1001-4942.2017.02.002

Abstract：The separation groups had been constructed using the national-level antigen material 8407 and the high susceptible and core germplasm material Guan 291 to Chinese cabbage TuMV as parents.In order to further verify the accuracy for detection of the molecular markers mBr4055 and BrID10723,which located on the nearest both sides of TuMV resistance gene TuRBCS01,107 individuals of F2 generation were selfed to construct the F2:3 families.The TuMV resistance of each F2:3 line were identified in order to determine the TuMV resistance and gene type of original F2 individuals.The 107 individuals of F2 generation were amplified using the above two markers.The marker detection accuracy was calculated according to the results of amplification and TuMV resistance.The results showed that 22 individuals were amplified both homozygous resistant bands in the two marker loci.Among them,2 individuals were proved to be heterozygous genotype by disease resistance test.The others were homozygous resistance genotype.The detection accuracy was 90.9％.And 48 individuals were amplified both heterozygous resistant bands in the two marker loci.Among them,5 individuals were proved to be homozygous resistant genotype,5 individuals were homozygous susceptible genotype by disease resistance test.The others were heterozygous resistant genotype.The detection accuracy was 79.2％.And 23 individuals were amplified both homozygous susceptible bands in the two marker loci.Among them,4individuals were proved to be heterozygous resistant genotype,one was homozygous resistant genotype by disease resistance test.The detection accuracy was 78.3％.The above detection results laid a foundation for molecular marker assisted selection using markers mBr4055 and BrID10723.