Cloning and Bioinformatic Analysis of PxALP1 Gene from Plutella xylostella
Hu Xia
Liu Xiaolin
Bai Yichuan
Xu Weihong
Liu Baiming
Zou Deyu
Xu Jingyang
Gu Xishu
Abstract：The diamondback moth,Plutdla xylostella (L.),is one of the world wide agricultural pests of cruciferous vegetables,and is also one of the earliest populations which produce resistance to pollution-free biological pesticide Bt toxin.The membrane bound alkaline phosphatase has been shown to be a direct receptor of Bt toxin protein in many insects according to previous studies.However,the coding genes and biological functions of Plutella xylostella alkaline phosphatase is rarely known.In this study,the open reading frame (ORF) of PxALP1 gene was cloned and the protein primary structure was deduced.A classic alkPPc (alkaline phosphatase homologues) domain was found in the 79th ～ 513th amino acid residue by SMART analysis,and a membrane bound domain was found through SWISS-MODEL homology model construction.Both of them indicated that PxALP1 was a potential membrane bound alkaline phosphatase.Phylogenetic analysis showed that diamondback moth PxALP1 and membrane combine alkaline phosphatase of Lepidoptera insect silkworm had the closest relationship.BLAST aligning exhibited that the identity between PxALP1 and silkworm BmAlpm was 36.3 ％ and the similarity was 50.3 ％,which proved that there was high conservation between them.
Keywords：Plutella xylostellaALP1Alkaline phosphataseBioinformatic analysis
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 7-12 )