Construction of Bivalent RNAi Expression Vector with Virus Resistance and Genetic Transformation of Hops
Wang Kun
Pei Juan
Li Yushun
Zhu Jianbo
Xiang Benchun
Abstract：To construct RNAi vector both resistant to Hop latent virus (HpLV) and Hop latent viroid (HpLVd),the pathogenic region of 202 bp in HpLV and the conserved sequence of 155 bp in HpLVd were selected as interference sequence through sequence alignment.The bivalent RNAi vector against HpLV and HpLVd were constructed based on interference vector pUCCRNAi,then its functional region was connected to plant expression vector pCAMBIA2300-35S-OCS through specific restriction sites.And the objective fragments were transformed into hops through Agrobacteriurn-mediated method.PCR identification showed that RNAi expression vector had been successfully transformed into hops.The construction of RNAi expression vector and Agrobacterium-mediated transformation in this study provided references to induce posttranscriptional gene silencing in transformed plants and obtain new multiple-virus-resistant plant materials.
Keywords：HopsVirus resistanceBivalent interference vectorGenetic transformation
Publication Date：2017-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )