Isolation,Identification and Biological Characteristics of a Wild Pleurotus sp.
Lan Yufei
Tang Lina
Li Xiumei
Wang Qingwu
Abstract：A fungi strain,XZG -ts,collected from the Mountain Tai,was isolated by tissue isolation and the internal transcribed spacers (ITS)was amplified by PCR.In the phylogenetic tree constructed with ITS,XZG -ts formed a branch with 3 isolates belonging to Pleurotus pulmonarius from Australian,India and China with the consistent rate of 100% in intra -group nucleotide.While it was only 98.10% ~99.06% with other 10 isolates of Pleurotus.Therefore XZG -ts was identified as P.pulmonarius.The effects of carbon source,nitrogen source,carbon -nitrogen combination and pH value on the growth of mycelium were further studied.The results showed the optimum carbon source,nitrogen source,temperature and pH value was fruc-tose,yeast extract,25℃ and 6,respectively.And the optimum carbon -nitrogen combination was 1% fruc-tose,1.0% maltose,0.2% yeast extract,0.3% peptone.
Keywords：Pleurotus sp.ITS sequence analysisBiological characteristicPleurotus pulmonarius
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 91-94 )