Effects of Different Explants and Plant Hormones on Callus Induction of Garlic
Abstract：The effects of different plant hormones on the callus induction of garlic were studied by using the base of clove,shoot tips,root tips and leaves of in vitro plantlet as explants in this study.The results showed that root tips and the base of clove were easier to induce callus than shoot tips and leaves of in vitro plantlet.And the callus induction rate of root tips was the highest in the four kinds of explants.Different plant hormone combinations had different effects on the four kinds of explants.The calli were induced from root tips in all combination of 6 -BA and 2,4 -D.The callus induction rate of the base of clove was 100% in the com-bination of 0.3 mg/L 6 -BA +1.5 mg/L 2,4 -D,or 0.5 mg/L 6 -BA +2.0 mg/L 2,4 -D.The callus induction rate of shoot tips was 85.83% in the best combination of 0.7 mg/L 6 -BA and 1.5 mg/L 2,4 -D. The combination of 0.3 mg/L 6 -BA and 2.0 mg/L 2,4 -D was the best for leaves of in vitro plantlet to in-duce callus,and the rate of callus induction was 71.67%.
Keywords：GarlicExplantPlant hormone combinationCallus
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 20-24 )