Morphological Characteristics of Pol CMS Line of Chinese Cabbage (Brassica rapa L.ssp.pekinensis)and Cloning and Expression Pattern Analysis of Related Sterility Genes
Abstract：Polima cytoplasmic male sterile (Pol CMS)is a very important CMS source in plant breeding. In the previous study,we used Pol CMS line KG -Pol of Chinese cabbage provided by Professor Ke Guilan as female parent and core line 92 -09 as recurrent parent to breed Pol CMS line 92 -09A of Chinese cabbage. Here,the morphological characteristics of Chinese cabbage Pol CMS line 92 -09A,its parental maintainer line 92 -09 and restorer line Zao 28 were investigated,and the Pol CMS gene Braraorf224 and its restorer gene BraraPPR were cloned and analyzed.The results showed that the petals,sepals,stamens and pistils of Chinese cabbage Pol CMS line 92 -09A were all smaller than those of its maintainer line 92 -09,and it could not form mature pollen with viability.While the maintainer line 92 -09 and the F1 hybrid of Pol CMS line 92 -09A ×restorer line Zao 28 both had pollen grains with viability.Bioinformatics analysis indicated that the nucleic acids and amino acids of Chinese cabbage Pol CMS gene Braraorf224 and restorer gene BraraPPR were very close to those of Brassica napus.The results of qRT -PCR showed that the expression level of BraraPPR in restorer line Zao 28 and F1 hybrid were both higher than that in Pol CMS line 92 -09A.The re-search would be helpful to understand the molecular mechanism of Pol CMS of Chinese cabbage in the future.
Keywords：Chinese cabbage (Brassica rapa L.ssp.pekinensis)Polima cytoplasmic male sterile (Pol CMS)Male -sterile geneRestorer gene
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：10( 6-15 )