Genomic DNA Extraction and CDDP Primer Screening of Rosa rugosa
Jiang Liyuan
Zang Dekui
Li Wenqing
Xie Xiaoman
Tong Boqiang
Abstract：DNA extraction of Rosa rugosa is difficult for their high contents of polyphenol and polysac-charide.Here a modified CTAB method for Rosa rugosa DNA extraction was presented through extending water -bath time,appropriately adjusting chloroform isoamyl alcohol (24∶1)extraction time to 2 ~3 times and etc.Twenty -one CDDP polymorphic primers were screened with reaction system containing 10 μL of 2 ×Es Taq MasterMix (including dye),1 μL of 40 ng/μL DNA template,1 μL of 10 pmol /μL primer and 8 μL of ddH2 O.The results showed that the genomic DNA of Rosa rugosa extracted with modified CTAB method owned high purity,and 16 CDDP primers had higher discriminating ability on Rosa rugosa.This research pro-vided bases for CDDP molecular markers and subsequent molecular biology researches of Rosa rugosa.
Keywords：Rosa rugosaModified CTAB methodDNA extractionCDDP molecular marker
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 13-17 )