Construction of A Maize CRISPR/Cas9 Vector
He Chunmei
Liu Tieshan
Guan Haiying
Liu Qiang
Dong Rui
Liu Chunxiao
Qi Shijun
Jia Xi
Wang Liming
Abstract：As its characteristics of high efficiency,easy manipulation and low cost,the clustered regular-ly interspaced short palindromic repeats (CRISPR)/CRISPR -associated (Cas)9 system has been widely used in precise modification of plant genome,which is regarded as a molecular tool with a broad application prospect.A detailed protocol of targeted mutagenesis of starch synthase regulatory factor gene ZmRSR1 in maize using the CRISPR/Cas9 system was provided,which might give a reference for site -directed modifica-tion of maize genome.
Keywords：CRISPR/Cas9MaizeGenome editingRegulation factor
Publication Date：2016-10-31
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )