Cloning and qRT -PCR Analysis of TaCPS1 Gene in Wheat
Guo Qingdong
Li Quanquan
Tian Qiuju
Qian Yanli
Xu Tian
Yang Yanlin
Wang Honggang
Feng Deshun
Abstract：ent -copalyl diphosphate synthase (CPS)is a key enzyme in the biosynthesis of phytoalex-ins.In this study,the cDNA of TaCPS1 gene which was related to resistance to powdery mildew was identified from the wheat -Thinopyrum alien addition line SN6306 by mRNA differential display.The cDNA had the length of 2 394 bp,which encoded 797 amino acid residues.Bioinformatics analysis indicated that the enco-ded protein which was mainly consisted of hydrophilic amino acids did not contain signal peptide.It was loca-ted in cytoplasm belonging to the Isoprenoid -Biosyn -C1 superfamily.qRT -PCR analysis showed that the expression of TaCPS1 gene in SN6306 was up -regulated about 45 times after induced by E09 for 72 hours, while that in YN15 was basically not induced.The expression of TaCPS1 increased by nearly 15 times and 2.4 times under the induction of JAMe and SA respectively.Thus we hypothesized that TaCPS1 might be in-volved in the regulation pathway of SA and JA in the powdery mildew resistance mechanism.
Keywords：Wheatent -copalyl diphosphate synthaseTaCPS1Gene cloningBioinformatics analy-sisFluorescent quantitative PCRPowdery mildew resistance
Publication Date：2016-07-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：9( 1-9 )