Identification of Molecular Markers for Clubroot Disease Resistant Gene CRb and Screening of Germplasm Resources of Chinese Cabbage
Ren Pingping
Ma Anfeng
Cheng Fei
Sun Zhaohui
Gao Jianwei
Abstract：Clubroot disease,caused by Plasmodiophora brassicae,is one of soil -borne disease seriously affecting the growth and development of Chinese cabbage (Brassica pekinensis L.)and has become one of the most serious diseases.Gene CRb is one of the most important gene against to clubroot disease in Chinese cab-bage.In this research,TCR05,an effective codominant marker closely linked to CRb,was identified from 6 published molecular markers.The marker owned a 279 -bp PCR amplification fragment in the resistant lines, a 250 -bp fragment in the susceptible lines and both 279 -bp and 250 -bp fragments in the heterozygous combinations.TCR05 was used to screen 24 germplasms.It was proved that they all contained gene CRb,8 were homozygous and the others were heterozygous.The results were in accordance with the field test results.
Keywords：Chinese cabbageClubroot diseaseCRbMolecular markerGermplasm resource
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 7-10 )