Differential Analysis and Functional Marker Development of Photoperiod Regulation Factor CCA1 from Two Chinese Cabbage Inbred Lines
Zhang Zhigang
Liu Shuantao
Li Qiaoyun
Wang Xiao
Wang Lihua
Zhao Zhizhong
Wang Shufen
Xu Wenling
Liu Xianxian
Liu Chen
Abstract：By the trend of photoperiod in spring and autumn,the sensibilities of two Chinese cabbage in-bred lines,06 -247 and He102,to photoperiod were identified.The 06 -247 was sensitive to long day, while He102 was opposite.The full -length cDNA sequence of photoperiod regulation factor CCA1 (Circadian clock associated 1 )from two lines were compared by homology -based cloning.It was revealed that there were two 6 -bp InDels and 14 non -synonymous SNPs between the CCA1 coding regions of 06 -247 and He102.The coding product had 552 and 556 amino acid residues respectively.The non -synonymous SNPs caused the exchange of amino acids with different property.The amino acid differences were mainly located in middle protein -protein interaction domain and C -terminal phosphorylation domain.The codominant molec-ular markers to distinguish the two 6 -bp InDels were developed and validated.The results provided founda-tion for further elucidating the molecular mechanism of photoperiodic response of CCA1 in Chinese cabbage.
Keywords：Chinese cabbageFlowering relying (not)on long dayBraCCA1Insertion /Deletion(In-Del)mutationFunctional marker
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )