Production Optimization of Biocontrol Bacillus subtilis B579 by Solid -State Fermentation Using Response Surface Methodology
Sun Bingbing
Yang Xiurong
Hao Yongjuan
Li Wei
Wei Jun
Tian Tao
Abstract：To enhance the fermentation level of biocontrol Bacillus,the solid -state fermentation condi-tions of Bacillus subtilis B579 were optimized using response surface methodology to establish the related tech-nology system.Firstly,the Plackett -Burman experiment was applied to screen the importan influential fac-tors,and then the path of steepest ascent was used to approach the biggest region of the cell yield.Moreover, the Box -Benhnken experimental design and response surface methodology were employed to get the regression model,which was proved through regression analysis and practice verification.The results showed that the three factors affecting fermentation production were beef extract,corn meal and soluble starch.The fermenta-tion production reached the highest as 1.93 ×10 11 cfu /g,when the content of beef extract,corn meal and sol-uble starch were 15.1,10.3 and 9.14 g/kg respectively.Under the optimized fermentation conditions,the solid fermentation production of biocontrol Bacillus increased by 15 times compared to the liquid fermentation. The regression model was verified to be accurate and reliable.
Keywords：Biocontrol BacillusSolid -state fermentationResponse surface methodology
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 62-66 )