Research on Tissue Culture of Korla Fragrant Pear
Liu Xiaofang
Feng Jianrong
Liang Xiaotong
LüWenjuan
Li Wenhui
Fan Xinmin
Abstract：Korla fragrant pear has soft flesh and delicious flavor,which is one of the important agricul-tural exports of China.While,there are problems of low fruit setting rate and unstable yield of Korla fragrant pear.Improving the productivity traits of fruit tree by genetic transformation based on tissue culture is a signifi-cant method for high efficiency breeding.In this paper,the in vitro tissue culture method of Korla fragrant pear was established using tender buds as explants.The effects of disinfection time of 0.1% HgCl2 solution on sterilizing effect and browning of explants were studied and the optimum multiplication medium and rooting me-dium were explored.The results showed that during the tissue culture process of Korla fragrant pear,the opti-mum disinfection time of 0.1% HgCl2 was 5 minutes;the optimum multiplication medium was MS +1.5 mg/L 6 -BA +0.1 mg/L IBA +1.5 mg/L GA3;and the optimum rooting medium was 1 /2MS +0.05 g/L AC +1.0 mg/L IBA.
Keywords：Korla fragrant pearBudTissue cultureDisinfection timeMultiplicationRooting
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 9-13 )