Clone,Bioinformatics Analysis and Protein Induced Expression of VDAC Gene in Feicheng Peach Fruits
Yi Guanshuai
Liu Yuchen
Zhu Shuhua
Abstract：Mitochondrial membrane permeability transition pore (MPTP)is a kind of protein complex, and plays important roles in cell mitochondria -mediated apoptosis.As an important part of MPTP,the volt-age -dependent anion channel protein (VDAC)regulates the opening of MPTP.However,its regulation mechanism in plant is still unclear.In this study,a cDNA encoding VDAC protein was isolated from Feicheng peach fruits by RT -PCR and rapid amplification of cDNA ends (RACE).The full -length cDNA of VDAC was 1 225 bp and contained 828 bp of coding sequence encoding a polypeptide of 276 amino acids.Multiple sequence alignment and phylogenetic tree analysis showed that the deduced protein VDAC of peach shared higher identify with that from other plants.The recombinant protein pET -30a -VDAC was constructed in vitro,expressed in Escherichia coli BL21,and purified through Ni -chelating affinity chromatography.The re-sults would lay a foundation for studying the structure and function of VDAC protein in peach fruits in the next step.
Keywords：Feicheng peachMitochondriaVoltage -dependent anion channel protein (VDAC)Gene cloneBioinformatics analysisProtein expression
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 1-7 )