Optimization of Preparation Technology for Special Phosphate Solubilizing Microorganism Agent in Brown Soil Tea Garden by Response Surface Methodology
Dong Yuhui
Zhang Lixia
Han Xiaoyang
Huang Xiaoqin
Zhou Bo
Abstract：The response surface methodology was used to optimize the preparation technology for special phosphate solubilizing microorganism agent in brown soil tea garden.Three key factors that affected the prepa-ration technology were screened out by Plackett -Burman design,including the ratio of water to carrier (X1 )、fermentation temperature (X4 )and drying temperature (X7 ).A quadratic regression equation model was es-tablished according to the steepest ascent experiment,central composite design and response surface methodol-ogy with the number of effective living bacteria in agent as response value.The maximum number of effective living bacteria predicted by this model was 5.01 ×10 10 per gram and the optimal preparation conditions were as follows:the ratio of water to carrier as 1.8,the amount of bacterial inoculation to carrier as 12.5% and the fermentation and drying temperature as 30℃ and 41℃ respectively.Under the optimization process condi-tions,the number of effective living bacteria in agent reached 5.19 ×10 10 per gram,which was basically con-sistent with the predicted maximum value.These results suggested that the prediction model was reliable.
Keywords：Tea gardenPhosphate solubilizing microorganism agentPreparation technologyResponse surface methodology
Publication Date：2016-03-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 56-61 )