Initial Establishment of Regeneration and Genetic Transformation System of Petunia Hybrid ‘Mambo Red’
Dong Yaru
Wang Zhaohong
Du Jianxun
Chen Chuanjie
Zhao Dongxiao
Abstract：Using petunia ‘Mambo Red’sterile leaves as explants,the regeneration system was estab-lished,then the genetic transformation system was set up on this basis.The results showed that the best medi-um to induce differentiation of leaf was MS +2.0 mg/L 6 -BA +0.1 mg/L NAA;the best rooting medium was 1 /2MS +0.1 mg/L NAA;the appropriate kanamycin screening pressure in the sprouting and rooting stage were 100 mg/L and 20 mg/L respectively;and the appropriate inhibitory concentration of cefotaxime was 200 mg/L.
Keywords：PetuniaMambo RedRegeneration systemGenetic transformation
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 14-17 )