Differential Analysis of MADS -box Genes during Dedifferentiation of Wheat Immature Embryo
Liu Haixia
Mo Haijiang
Cheng Yuhong
Ma Hongbo
Xia Haidong
Hong Wei
Dong Hongxing
Chen Junying
Abstract:The expression profiles of MADS -box genes during dedifferentiation of wheat immature em-bryo were firstly analyzed using wheat gene chip technique.Total 20 MADS -box genes were detected,among which,14 genes were down -regulated and 6 genes presented mixed expression.Down -regulated expression of most of these MADS -box genes indicated that they might have strong inhibitory effects during the differenti-ation of wheat immature embryo.Besides,the 12th hour was a key expression period of these MADS -box genes.The expression profiles of 4 genes,including CA670406,CK213813,CA608865 and AB107992, were verified by semi -quantitative RT -PCR technique,which were basically consistent with those of wheat gene chip.
Keywords:WheatMADS -box genesGene chipImmature embryoDedifferentiation
Publication Date:2016-03-01
Online Publishing Date:2026-05-22(First online date of this platform, not the publication date of the document)
Pages:5( 9-13 )
