Prediction and Analysis of GS -Elong Genes in Brassica oleracea L.
Song Jia
Wang Jue
Liu Lei
Wang Hui
Abstract：Using the full CDS and protein sequences of Arabidopsis glucosinolate biosynthesis related GS -Elong gene family as references,the candidate homologous genes with Score≥100,E -value≤10 -10 and Coverage ≥70% were identified from genome database of Brassica oleracea L.through BLASTN and BLASTP.Then,the homology and structures of these genes were compared and the clustering analysis was conducted.The results indicated that there were four candidate GS -Elong genes (Bol020647,Bol017070, Bol037823 and Bol017071)in Brassica oleracea.The homologous GS -Elong genes in Brassica oleracea and Arabidopsis shared 66.1% ~84.3% nucleotide sequence identity,and four paralogs GS -Elong genes in Brassica oleracea shared 62.0% ~83.7% nucleotide sequence identity.The Brassica oleracea and Arabidopsis GS -Elong genes were highly consistent on the number and size of exon,but the two Brassica oleracea GS -Elong genes,Bol017071 and Bol037823,had three and one exon less respectively than those of Arabidopsis. The phylogenetic and collinearity analyses showed that there were two BoMAM1 (Bol017070 and Bol020647) and two BoMAM3 (Bol017071 and Bol037823)in Brassica oleracea.
Keywords：Cruciferous plantsBrassica oleracea L.GlucosinolatesGS -Elong
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )