Sequence Analysis on rDNA -ITS Region of Germplasm Resources from Juglans
Xu Li
Chen Xin
Wei Hairong
Zhang Lisi
Zong Xiaojuan
Wang Jiawei
Zhu Dongzi
Tan Yue
Liu Qingzhong
Abstract：The internal transcribed spacer (ITS)of 9 different germplasm resources from Juglans were amplified and sequenced with designed primers.Using Dnasp 5.0 and MEGA 6.0,the sequence characteris-tics and different sites were analyzed,and the phylogenetic tree was performed.The length of ITS sequences ranged from 644 to 647 bp.There were 48 polymorphic sites,and the transition to transversion ratio was 1.75.The phylogenetic tree suggested that there was close genetic relationship between J.cathayensis Dode and J.mandshurica Maxim.,and then they were clustered into one group with J.hopeiensis Hu,which was consistent with traditional taxonomy.The results provided theoretical basis for utilization of germplasm re-sources of Juglans.
Keywords：JuglansITS regionSequence analysis
Publication Date：2016-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )