Pathotypes and Virulence Analysis of Verticillium dahliae in Shandong Province
Wei Chunzhi
Liu Zhaoxia
Wu Guiyuan
Wang Zhimin
Shen Fafu
Liu Aixin
Abstract：Verticillium wilt is one of the most destructive diseases of cotton in Shandong Province.The samples of diseased cotton plants were collected from Dezou,Dongying and Taian,and 202 strains of Verticilli-um dahliae Kleb.were isolated and identified.The primers of INTD2f/INTD2r and INTND2f/INTND2r, which were specific for identifying defoliating (D)and non -defoliating (ND)pathotype respectively,were used to amplify the specific segment by PCR.At the same time,8 strains were randomly selected out to ana-lyze their toxin secretion ability and wilt index.The results showed that 201 strains were identified as D patho-type,and only 1 strain was identified as non -defoliating (ND)pathotype.The D pathotype of strain HDF4 -3 -1 showed the highest toxin secretion ability with the content of toxin as 50.82 μg/mL,however,the ND pathotype of strain HLX5 -1 -1 had lower toxin secretion ability with the toxin content as only 13.85 μg/mL. The wilt index of D pathotype of strain HDF5 -3 -1 was the highest as 72.5%,while that of ND pathotype of strain HLX5 -1 -1 was the lowest as 42.5%.In a word,D pathotype of strains had higher toxin secretion a-bility and wilt index,overall.
Keywords：Verticillium dahliaeDefoliating pathotypeNon -defoliating pathotypeToxin secretion a-bilityWilt index
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 80-84 )