Cloning and Specific Marker Development of TaPAPA1 Gene in Wheat
LGuangde
Gao Minggang
Li Hongna
Guo Ying
Li Sishen
Abstract：Zinc -finger HIT protein plays an important role in the regulation of plant defense and stress response.In this study,a gene named TaPAPA1,who encoded one protein containing PAPA -1 -like [Pim -1 -associated protein -1 (PAP -1 )-associated protein -1 -like]and Zf -HIT (Zinc -finger HIT)domain, was cloned from wheat.Through sequence analysis,three cDNA and three gDNA sequences were got,which all had six exons and five introns.According to different sites among three gDNA sequences,three pairs of genome special primers were designed,and these genes were located in homologous group 5 using nullisomic -tetrasomic lines of Chinese Spring.The coding sequences of TaPAPA1 -5A,TaPAPA1 -5B and TaPAPA1 -5D were 1 473,1 473 bp and 1 470 bp,which encoded polypepetides with 490,490 and 489 amino acids,respectively. According to the SNP locus in TaPAPA1 -5B,the AS -PCR marker TaPAPA1 -5B -AS1 /2 was developed.U-sing a RIL (recombinant inbred line)population,the marker was located between marker D -1207571 and D -1100783 on 5B by linkage analysis with the genetic distance of 0.51 cMand 0.71 cM,respectively.
Keywords：Wheat (Triticum aestivum L.)TaPAPA1Zf -HIT proteinSNP siteMarker develop-mentLinkage analysis
Publication Date：2015-11-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 12-17 )