Construction of HIGS Vectors for Laccase Genes from Aspergillus flavus and Its Transformation to Peanut
Sun Quanxi
Wang Yunyun
Wang Xiuzhen
Tang Yueyi
Wu Qi
Zhang Qingyun
Cao Guangying
Wang Chuantang
Abstract：Aflatoxin contamination is a severe problem adversely affecting peanut food safety.Breeding a new peanut variety resistant to Aspergillus flavus is a lengthy process through conventional means,and the re-sults are far from satisfaction.Host -induced gene silencing (HIGS),as a novel technology for RNA interfer-ence,provides possibilities for creating disease -resistant crop plants.However,there is no reports on pea-nut.To breed new A.flavus -resistant peanut through genetic engineering,the HIGS vectors containing 2 lac-case genes,Lac1 and Lac2,were constructed and then were transformed into an A.flavus -susceptible peanut cultivar Yueyou 20.PCR -positive transgenic peanut seeds with Lac1 gene were obtained.According to the HIGS principle,if the transgenic peanut is infected by A.flavus,the laccase gene expression will be inhibited by dsRNA,thus the resistance of peanut to A.flavus will be induced.Based on this principle,whether the transgenic seeds had resistance to A.flavus infection would be identified next.The study layed foundations for exploring the relationship between laccase gene and A.flavus pathogenicity in peanut,and provided a new way for breeding peanut cultivars with A.flavus resistance.
Keywords：PeanutAspergillus flavusLaccase geneHost -induced gene silencing (HIGS)Genetic transformation
Publication Date：2015-10-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 8-12 )