Screening and Identification of a High -Efficient Cellulose -Degrading Bacterium and Determination of Its Enzyme Activity
Liu Lu
Gong Chunyan
Li Li
Zhao Hongtao
Abstract：The corn straw stack samples in Jiaonan were used as bacterium sources to isolate and screen high -efficient cellulose -degrading bacteria,and then they were identified and their enzyme activities were de-termined.The results showed that ten cellulose -degrading strains were isolated through enrichment culture with corn straw as the only carbon source and the method of Congo red decolorization.One strain 0901 was screened out with higher production of cellulase by comparing the size of transparent circle.The enzyme activity of 0901 was tested as 1 267.43 U /mL through the method of 3,5 -dinitrosalicylic acid colorimetry (DNS)colorimetric method.According to the results of sequence alignment,the phylogenetic tree was constructed.Combined with the analysis of morphological,physiological and biochemical characteristics,0901 was identified as Bacillus sub-tilis.Its degradation effect on corn straw was verified.The weight loss rate of corn straw after 30 days was 36.2%.It indicated that the Bacillus subtilis strain 0901 had higher cellulose degradation ability.
Keywords：CelluloseDegradation abilityScreenIdentificationCellulase activity
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 64-68 )