Cloning and Bioinformatics Analysis of Pathogenic Protein CYP51C cDNA in Fusarium graminearum
Sun Xiaomei
Huang Jinguang
Abstract：In this study,the full -length cDNA sequence of pathogenic protein CYP51C from Fusarium graminearum was cloned using RT -PCR,and the bioinformatics analysis of amino acid sequences was con-ducted and its typical characteristics was identified.The results showed that the full -length cDNA sequence of CYP51C included a complete open reading frame (ORF)of 1 554 bp,which encoded 517 amino acids.Its molecular weight was 58.6 kD and its isoelectric point was 6.36.It possessed the conserved domains which were characteristics of cytochrome P450 family members.It was not a secretory protein without a signal pep-tide,but was a hydrophilicity protein with transmembrane domain.The major structural elements of polypep-tide chain were α-helix and random coil.Subcellular localization result indicated that CYP51C was mainly located in cytoplasm.These results would provide references for the study on protein purification and protein structural biology,and then lay a foundation for drug design targeting pathogenic fungi.
Keywords：Fusarium graminearumSterol 14α-demethylation(CYP51)Gene clonePathogenicityBioinformatics analysis
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：6( 1-6 )