Analysis on DNA Extraction Effect from Cotton Leaves under Different Storage Conditions
Zheng Zipiao
Qu Yanying
Wang Lili
Zhang Yanan
Wang Yajun
Wei Jing
Gu Aixing
Abstract：This study was conducted based on the problems that inappropriate storage could lead to poor extraction rate and purity of DNA from cotton leaves.With the fresh leaves as control,the DNA extraction effects of cotton leaves were compared after storage in five kinds of conditions to screen out a best storage meth-od.The results showed that the DNA extraction rate of cotton leaves was the highest after -20℃ frozen stor-age for 3 days with better purity,which increased by 51.4% compared to that of the control.The DNA yield of 3 -day frozen storage was obviously higher than that of 3 -day 4℃ storage,and the 7 -day liquid nitrogen frozen had clear advantages over the silica gel dry storage and 7 -day frozen storage.The DNA purity of 7 -day liquid nitrogen frozen was close to that of 7 -day silica gel dry storage.In conclusion,-20℃ frozen storage could be used for short -term storage,while the liquid nitrogen frozen could be used for longer -time storage.
Keywords：Storage conditionCotton leavesDNA extractionPurityExtraction rate
Publication Date：2015-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：3( 11-13 )