Cloning and Sequence Analysis of PHR1 Gene from Leymus mollis and Thinopyrum bessarabicum
Zhang Xueying
Li Taiqiang
Wang Honggang
Li Xingfeng
Abstract：To fully explore the plant potential for high -efficiency phosphorus utilization and enhance the plant absorption and use efficiency of insoluble phosphorus in soil will play important roles in breeding new va-rieties,reducing fertilizer input and protecting agro -ecological environment.PHR1 is an important transcrip-tion factor of multiple Pi starvation responses in Pi signaling pathways.By homologous cloning,two PHR1 genes were cloned from Elymus mollis and Thinopyrum bessarabicum,which were named as LmPHR1 and Tb-PHR1.Their ORFs were both 1 356 bp,and both of them encoded 451 amino acids;their cDNA sequences shared 98.63% identity with the cDNA sequences of Xiaoyan 54 PHR1 -A1,PHR1 -B1,PHR1 -D1.Their encoded proteins both had a MYB DNA binding domain and a predicted coiled -coil domain.Using the Clust-alX for phylogenetic analysis of the two PHR1 genes with the reported PHR1 genes in rice and wheat,ect.The results showed that PHR1 had closer evolutionary relationship with the PHR1s from Brachypodium distachyon, Oryza sativa,Zea mays and Arabidopsis thaliana;while the LmPHR1 had more faraway evolutionary relation-ship with other plants’PHR1 genes.
Keywords：Leymus mollisThinopyrum bessarabicumPHR1 geneHomologous clone
Publication Date：2015-08-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 1-5 )