Development of a Molecular Marker Identifying Cytoplasmic Male Sterility Gene in Onion(Allium cepa L.)
Li Yongbo
Liu Bingjiang
Huo Yumeng
Miao Jun
Yang Jianping
Wu Xiong
Yang Yanyan
Abstract：The specific primers of orfA501 in chives and cytoplasmic male sterile line of onion were used to PCR amplification.The amplified fragment was extracted and sequenced.The primers were designed ac-cording to the obtained sequence for genome walking,and partial flanking sequence was obtained.Then a SCAR marker was developed and named as OC2175.With this marker,a special fragment of 2 175 bp could be obtained from the male sterile lines and a 1 053 bp fragment was amplified from both (s)-cytoplasm and (N)-cytoplasm.Seventeen pairs of male sterile lines and their corresponding maintainer lines with different genetic backgrounds were used to verify this SCAR marker.The results indicated that the SCAR marker was perfectly suitable for distinguishing the cytoplasm types S or N in onion.
Keywords：OnionCytoplasmic male sterilityMolecular marker
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )