Fluorescence In Situ Hybridization Analysis of Hordeum chilense Chromosomes
Abstract：In order to develop new cytogenetic markers specific for Hordeum chilense chromosomes , the Chinese Spring-H.chilense amphipliod, heterozygous 1Hch﹠1HchS additon, 4Hch, 5Hch, 6Hch and 7Hch ad-ditions were analyzed by fluorescence in situ hybridization (FISH) using Oligo-pTa535.1, Oligo-pSc119.2 and ( GAA) 8 as probes .The results of double-color FISH using Oligo-pTa535 .1 and Oligo-pSc119 .2 as probes showed that the individual 1Hch ~7Hch chromosomes were painted by different signals except 3Hch chro-mosomes with Oligo-pSc119 .2 probe;the FISH using ( GAA) 8 as probe suggested that most of subtelomeric and centromere regions of 1Hch ~7Hch chromosomes were labeled by hybridization signals .The FISH signals of H.chilense chromosomes using these three probes were different from those of wheat chromosomes , and no oli-gonucleotide sequence elimination occurred during chromosome transformation .Therefore , FISH with these three probes could be used to identify H.chilense chromosomes in wheat background and be assistant to wheat breeding by chromosome engineering using the agronomical useful genes of H.chilense.
Keywords：Hordeum chilenseAddition lineChromosomeFISHCytogenetic marker
Publication Date：2015-05-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：5( 10-14 )