Optimization of Sporulation Fermentation Process of Bacillus subtillus NS178 by Response Surface Methodology
Wang Xixiang
Ding Yanqin
Du Binghai
Yao Liangtong
Qi Guozhen
Ge Ke
Liu Kai
Abstract：Three factors mainly influencing the colony number of endophytic Bacillus subtillus NS178 in fermentation liquid were screened out by single-factor experiments, which were glucose, ( NH4 ) 2 SO4 and K2 HPO4 .The Box-Behnken Design was applied to optimize the fermentation medium for NS178, and the op-timum one was obtained as 2.29%glucose, 0.3%(NH4)2SO4 and 0.23%K2HPO4 after the analysis of re-sponse surface methodology.The culture conditions were also optimized, and the optimal conditions were tem-perature as 33℃, pH value as 7.6 and fluid volume as 50 mL.Under the optimal fermentation conditions, the yield of NS178 was 34.50 ×108 cfu/mL;the spore rate could reach 75.8%after cultured for 72 hours;and the diameter of inhibition zone increased by 67%compared to that before fermentation.
Keywords：Bacillus subtillusSporeResponse surface methodologyInhibition zone
Publication Date：2015-04-02
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：7( 59-65 )