Acquisition and Bioinformatics Analysis of gsp G and gsp K Genes of Ralstonia solanacearum from Mulberry
Zhuo Su
Kong Weiqing
Abstract：gsp G and gsp K genes are involved in the formation of periplasmic complex of Ralstonia so-lanacearum type Ⅱ secretion system .The sequences of gsp G and gsp K genes of Ralstonia solanacearum strain MR111 with 438 bp and 843 bp in length respectively were obtained from mulberry by PCR amplication and DNA sequencing technology .By online BLAST analysis , the similarity of the 2 genes with the homologous genes from other R.solanacearum species were both over 90%.There were typical functional structure of T2SG and T2SK, and secretory signal peptide composed mainly by leucine and alanine in the N -end in the 2 genes, respectively.A typeⅣsecretion system “Ⅳ_pilin_GFxxxE” structure was found in gsp G, and a re-peat sequence “GIQSTE” was found in gsp K.This repeat sequence was highly conserved in R.so-lanacearum.
Keywords：MulberryRalstonia solanacearumType Ⅱsecretion systemgsp gene
Publication Date：2015-01-01
Online Publishing Date：2026-05-22(First online date of this platform, not the publication date of the document)
Pages：4( 9-12 )